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A history of preterm birth, both with and without a history of BPD, has been consistently associated with poorer lung function in later life 4 , and there is growing evidence that those with a history of BPD risk the development of chronic obstructive pulmonary disease (COPD) in early adulthood 5, 6 , as well as being diagnosed with asthma 7 , although there is increasing recognition that prematurity-associated lung disease (PLD) has different underlying mechanism to asthma 8

The membranes were incubated with the primary antibodies provided in each ELISA kit at the concentrations (and diluents) suggested by the manufacturer overnight and washed 3 times in TBS-T (5 min per wash)

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