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176 Mice lacking ZIP4 exhibit disrupted villus integrity, highlighting the significance of ZIP4 in preserving the architecture of the intestinal epithelium

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In cell culture studies, concentrations of 10 to 1,000 nanomolar (nM) are used, with the physiological range at approximately 200 nM

After rinsing, slides were incubated with the secondary antibody Alexa Fluor 488 goat anti-rabbit (A-11008 Invitrogen - Thermo Fisher Scientific) or Alexa Fluor 488 donkey anti-goat (A-11055 Invitrogen - Thermo Fisher Scientific) 1:500 in PBS 1 0.5% BSA 0.1% saponin (w/v/v) for 2 h at room temperature

Frequency : How often the peptide is administered (e.g., 1x daily, 2x weekly)