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Lee SH, Min SH, Cho YC, Han JH, Kim MN, Kim CR, et al

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In addition, we showed that GSH depletion induces autophagy and SIPS, with autophagy being a negative regulator of SIPS

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Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution