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In order to avoid further HIF-1 degradation during whole cell lysates preparation, cells were lysed directly with NuPAGE LDS Sample Buffer (1X) (Thermo Fisher Scientific, San Jose, CA, USA) in the presence of protease and phosphatase inhibitors cocktail (Cell Signaling Technology, Danvers, MA, USA), similarly to ref

Sample size = 25 for each mercury vapor exposure during dental student training in Amalgam removal

However, some studies show that ASNS levels may not impact the sensitivity of ALL to asparaginase treatments in all cases [98]

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This involves less frequent administration to sustain the results without continuously up-regulating the system