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After each cycle, a 4-month rest is required before starting the next one, with a maximum of 3 cycles per year

HepG2 cells were treated with 5 mol/ml (Figure 1A) and 10 mol/ml (Figure 1B) of ASA for 48 h and GSH levels in the mitochondria and post mitochondrial supernatant (PMS) were measured by enzymatic method as described in the Materials and Methods

Lyophilized peptides remain stable at room temperature for short periods but should be refrigerated for long-term storage

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The research versus pharmaceutical grade comparison also matters here, because pharmaceutical-grade formulations often come pre-reconstituted at standardized concentrations, eliminating the calculation step entirely