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The EB dye content was calculated using the following formula: Measurement of mean arterial pressure (MAP) Systolic and diastolic blood pressure was recorded by the tail-cuff technique using an NIBP ML125 system (ADInstruments, Australia.blood pressure was measured by placing animals tail in the cuff of a monometer

Nutritional assessment: Completed by a registered dietitian or qualified clinician, documenting caloric requirements and inability to meet them orally

J., 113, 123-130, (1969)

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