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Sample preparation for label-free quantitative MS Cells were lysed by addition of lysis buffer (8 M urea, 50 mM NaCl, 50 mM EPPS pH 8.5 and protease and Phosphatase inhibitors) and homogenization by bead beating (BioSpec) for three repeats of 30 s at 2,400 strokes per min

#2217), total IGF-1R (1:1000

Zhang W, Li Z, Li T, et al

This limitation is particularly evident in understanding the roles of GRX, GSNO, S-glutatylation, and S-nitrosylation processes