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The cells were exposed for various times, after which the media was removed and the cells were washed with warm PBS and lysed by brief sonication in fresh PBS
& Muckenthaler, M
Briefly, the brain homogenate was diluted 1:20 (v:v) with ice-cold Lockes buffer (154 mM NaCcl, 5.6 mM KCl, 3.6 mM NaHCO3, 2.0 mM CaCl2, 10 mM D-glucose and 5 mM HEPES, pH 7.4) to obtain a concentration of 10 mg tissue/ml
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