The slides were washed in water/PBS and then blocked using 5% goat serum in PBS for 1 h at room temperature prior to adding primary antibodies diluted in the blocking buffer
Pericapillary haem-rich deposits: evidence for microhaemorrhages in aging human cerebral cortex
The millimolar GSH intracellular concentrations, the low plasma micromolar concentrations and the low GSH redox potential ( E 0 = 240 mV) make GSH an ideal and perfect cellular redox buffer (Figure 2) ( FIGURE 1 FIGURE 2 Glutathione is synthesized in the cytosol of all cells from their precursor amino acids: glutamic acid, cysteine and glycine by consecutive action of two enzymes: -glutamyl-cysteine (-GluCys) synthetase (also known as glutamate cysteine ligase, GCL) that in a first step uses glutamate and cysteine as a substrate to form the dipeptide -glutamyl-cysteine
Glutathione and glutathione conjugate efflux from cultured liver cells
British Journal of Clinical Pharmacology, 56 (6), 635638
Navarro J, Obrador E, Pellicer JA, Aseni M, Via J, Estrela JM