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A family of NADPH/NADP + biosensors reveals in vivo dynamics of central redox metabolism across eukaryotes

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Use 5-10 bed volume of Elution buffer (added GSH) to elute, collect in fractions, collect one tube for each bed volume, and test separately, which can not only ensure that all binding target proteins are eluted, but also obtain high-purity and high-concentration proteins

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Cell Mol Life Sci 72:13311345 Sadoshima J, Montagne O, Wang Q, Yang G, Warden J, Liu J, Takagi G, Karoor V, Hong C, Johnson GL, Vatner DE, Vatner SF (2002) The MEKK1-JNK pathway plays a protective role in pressure overload but does not mediate cardiac hypertrophy

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