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Statistical significance was assessed by an unpaired two-tailed t-test (Control, n = 17

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150 L of 0.2 mol/L pH 7.8 phosphate buffer, 50 L of proxy matrix, and 10 L of 0.001 mol/L N-Hcy-Lys were mixed with 5 L of a mixture, prepared by mixing 0.2 mol/L BCPB solution and 0.25 mol/L TCEP solution in volume ratio 1:1

This is the opposite timing from cognitive peptides like Semax, which we administer in the morning to take advantage of its focus-enhancing effects during active hours

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