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The ipratropium-bound structure demonstrates how OCTN2 binds positively charged drugs We selected the anticholinergic bronchodilator, ipratropium, as a model compound to investigate how OCTN2 binds positively charged drug molecules

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In contrast, strong oxidative conditions induced by HO resulted in irreversible oxidation

Practically it provides a convenient N-terminal leader for bacterial expression, which is part of why this construct became the standard reagent rather than a laboratory curiosity