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According to Lindgren, it takes 3-6 months to see an upregulation, or increase, in the overall amount of GH isolates being produced by the pituitary gland

The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48

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