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GFAP is an intermediate filament protein commonly used as a marker for astrocytes, which significantly increases in pathological-like states (e.g., aging and MS), indicating heightened astrocyte reactivity in rodents and humans (Nichols et al., 1993

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Finally, the samples were brought to dryness using an evaporation system (TurboVap LV, Biotage, Uppsala, Sweden) and resuspended in 100 L methanol-water (50/50, v/v) for UPLC-MS/MS automated injections